Leukemia Inhibitory Factor
Leukemia Inhibitory Factor (LIF) is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinegric neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo.
picture: LIF (hBA): sc-4988. Undifferentiated mouse M1 cells cultured for 2 weeks in 0,3% agar medium without LIF (A) and differentiated mouse M1 cells cultured for 2 weeks in 0,3% agar medium with 1ng/ml of human LIF (B).
Products:
sc-4988 LIF (hBA) human
* mature leukemia inhibitory factor (LIF) of human origin, lyophilized
* available as 10^6 units (10 µg) (sc-4988) or 5x10^6 units (50 µg) (sc-4988A)
* potent inhibitor of spontaneous embryonic mouse and human stem cell differentiation and useful for in vitro maintenance of the pluripotential phenotype of ES cells; equal activity on both human and mouse cell lines
* 1000 U per 1 ml of tissue culture media is required to maintain ES cells with a stem cell phenotype; 10^6 units is sufficient for 1 L of tissue culture media, 10^7 units is sufficient for 10 L of tissue culture media
* produced in E. coli as a GST-tagged fusion protein; GST tag is cleaved and LIF is purified by HPLC chromatography
* >95% pure by SDS-PAGE and 0.22 micron sterile filtered prior to lyophilization; tested negative for mycoplasmas
* Mature, biologically active, recombinant LIF is indistinguishable from native LIF in its biological activities in vitro.
* Leukemia Inhibitory Factor (LIF) is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinergic neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo.
sc-4989 LIF (mBA) mouse
* mature leukemia inhibitory factor (LIF) of mouse origin, lyophilized
* available as 10^6 units (10 µg) (sc-4989) or 5x10^6 units (50 µg) (sc-4989A)
* potent inhibitor of spontaneous embryonic mouse stem cell differentiation and useful for in vitro maintenance of the pluripotential phenotype of ES cells
* 1000 U per 1 ml of tissue culture media is required to maintain mouse ES cells with a stem cell phenotype; 10^6 units is sufficient for 1 L of tissue culture media, 10^7 units is sufficient for 10 L of tissue culture media
* produced in E. coli as a GST-tagged fusion protein; GST tag is cleaved and LIF is purified by HPLC chromatography
* >95% pure by SDS-PAGE and 0.22 micron sterile filtered prior to lyophilization; tested negative for mycoplasmas
* Mature, biologically active, recombinant LIF is indistinguishable from native LIF in its biological activities in vitro.
* Leukemia Inhibitory Factor (LIF) is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinergic neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo.
