ß-catenin (clone E247 - Rabbit Monoclonal)
ß-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation. ß-catenin is phosphorylated at four serine and threonine residues at the N-terminus. Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33 . Mutations in the -catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas.
AN510-5M ß-catenin (RTU Manual), 6ml cena 7.551 Kč
AN510-10M ß-catenin (RTU Automated), 10ml cena 13.406 Kč
NU510-UC ß-catenin (Concentrated), 1ml cena Volejte Kč
CD99 (clone EPR3097Y - Rabbit Monoclonal)
CD99, or Protein MIC2, is a T-cell surface glycoprotein involved in the T-cell adhesion process and spontaneous rosette formation with erythrocytes. The cell surface antigen defined by the monoclonal antibody CD99 is expressed by both the human X and Y chromosomes; the gene loci on the X and Y chromosomes are referred to as MIC2X and MIC2Y, respectively. It was demonstrated that engagement of distinct epitopes on CD99 rapidly induces T cell death by a novel caspase-independent pathway. Thus, it is suggested that CD99 is linked to a novel death pathway that may have biologic relevance in control of early T cells.
AN518-5M CD99 (RTU Manual), 6ml cena 7.551 Kč
AN518-10M CD99 (RTU Automated), 10ml cena 13.406 Kč
NU518-UC CD99 (Concentrated), 1ml cena Volejte Kč
Factor XIII A (clone EP3372 - Rabbit Monoclonal)
Factor XIII (plasma transglutaminase, fibrin stabilizing factor) is a plasma protein that plays an important role in the final stages of blood coagulation and fibrinolysis. Factor XIII A is activated by thrombin and calcium ion to a transglutaminase that catalyzes the cross linking of fibrin molecules, forming intermolecular isopeptide bonds, thus stabilizing blood clots. In two diseases that share some histological resemblance (Lymphocyte-poor graftversus-host-reaction and toxic epidermal necrolysis), Factor-XIII A-positive dendrocytes show some morphological changes, probably as a response to altered cytokine environment. Factor-XIII A-positive dendrocytes thus are reported to possibly play a role in the regulation of the connective tissue remodeling that may accompany epidermal destruction.
AN516-5M Factor XIII A (RTU Manual), 6ml cena 8.793 Kč
AN516-10M Factor XIII A (RTU Automated), 10ml cena 15.580 Kč
NU516-UC Factor XIII A (Concentrated), 1ml cena Volejte Kč
Paxillin (N-Term) (clone Y113 - Rabbit Monoclonal)
Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Paxillin plays a significant role in integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization. Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration.
AN521-5M Paxillin (N-Term) (RTU Manual), 6ml cena 10.346 Kč
AN521-10M Paxillin (N-Term) (RTU Automated), 10ml cena 15.580 Kč
NU521-UC Paxillin (N-Term) (Concentrated), 1ml cena Volejte Kč
Transcription Factor PU.1 (clone EPR3159Y - Rabbit Monoclonal)
Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages.
It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Transcription factor PU.1 and AML1, a DNA-binding subunit of the CBF transcription factor family, act as tumor suppressors in leukemia. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation. The lack of transcription factor PU.1 protein expression in classical Hodgkin’s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD.
AN520-5M Transcription Factor PU.1 (RTU Manual), 6ml cena 8.882 Kč
AN520-10M Transcription Factor PU.1 (RTU Automated), 10ml cena 13.406 Kč
NU520-UC Transcription Factor PU.1 (Concentrated), 1ml cena Volejte Kč
Cyclin E2 (clone EP454Y - Rabbit Monoclonal)
Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both. Abnormally high levels of cyclin E expression have frequently been observed in human cancers.
AN519-5M Cyclin E2 (RTU Manual), 6ml cena 7.551 Kč
AN519-10M Cyclin E2 (RTU Automated), 10ml cena 13.406 Kč
NU519-UC Cyclin E2 (Concentrated), 1ml cena Volejte Kč
NGF Receptor (clone EP1039Y - Rabbit Monoclonal)
Neurotrophins signal cell survival, differentiation, growth cessation, and apoptosis through two classes of cell surface receptors, the Trks and NGF Receptor (p75NTR). p75NTR, when it is not bound by NGF, may play a role in neuronal selection during embryonic development and suggest that neuroblastomas may arise from immature neuroblasts that escape programmed cell death. Therefore, the loss of p75NTR expression in developing neural crest cells might be a primary event in the genesis of neuroblastoma. It has also been suggested that neuronal death in Alzheimer's disease is mediated, at least in part, by the interaction of beta-amyloid with p75NTR, and suggest new targets for therapeutic intervention.
AN522-5M NGF Receptor (RTU Manual), 6ml cena 10.346 Kč
AN522-10M NGF Receptor (RTU Automated), 10ml cena 15.580 Kč
NU522-UC NGF Receptor (Concentrated), 1ml cena Volejte Kč
PDCD4 (clone EPR3432 - Rabbit Monoclonal)
Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits tumor promoter-induced neoplastic transformation. It down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. PDCD4 inhibits the translation initiation factor eIF4A, an RNA helicase that catalyzes the unwinding of secondary structure at the 5' untranslated region (5'UTR) of messenger RNAs (mRNAs). It is also believed that regulated degradation of PDCD4 in response to mitogens allows efficient protein synthesis and consequently cell growth.
AN524-5M PDCD4 (RTU Manual), 6ml cena 7.551 Kč
AN524-10M PDCD4 (RTU Automated), 10ml cena 13.406 Kč
NU524-UC PDCD4 (Concentrated), 1ml cena Volejte Kč
CD14 (clone EPR3653 - Rabbit Monoclonal)
CD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes.
AN515-5M CD14 (RTU Manual), 6ml cena 7.551 Kč
AN515-10M CD14 (RTU Automated), 10ml cena 13.406 Kč
NU515-UC CD14 (Concentrated), 1ml cena Volejte Kč
p120 Catenin (clone YE372 - Rabbit Monoclonal)
Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. p120 Catenin is one member of this Armadillo gene family of junctional plaque proteins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Recently it became clear that some catenins also mediate nuclear signaling. p120 Catenin is implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.
AN523-5M p120 Catenin (RTU Manual), 6ml cena 8.793 Kč
AN523-10M p120 Catenin (RTU Automated), 10ml cena 15.580 Kč
NU523-UC p120 Catenin (Concentrated), 1ml cena Volejte Kč
Caldesmon (clone E89 - Rabbit Monoclonal)
Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin. Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the Nterminus. Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin.
AN507-5M Caldesmon (RTU Manual), 6ml cena 8.793 Kč
AN507-10M Caldesmon (RTU Automated), 10ml cena 15.580 Kč
NU507-UC Caldesmon (Concentrated), 1ml cena Volejte Kč
CD8 (clone EP1150Y - Rabbit Monoclonal)
The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as a disulfidelinked homodimer of two CD8alpha monomers, or a heterodimer of CD8alpha and CD8beta. These receptors interact with ligands, such as major histocompatibility complex (MHC) class I, on the outside of the cell, with proteins inside the cell, such as the tyrosine kinase p56lck, and possibly with proteins on the same cell-surface. CD8 plays an important role in T cell development in the thymus and T cell activation in the periphery.
AN513-5M CD8 (RTU Manual), 6ml cena 7.551 Kč
AN513-10M CD8 (RTU Automated), 10ml cena 13.406 Kč
NU513-UC CD8 (Concentrated), 1ml cena Volejte Kč
CD5 (clone EP2952 - Rabbit Monoclonal)
CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation. CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5.
AN512-5M CD5 (RTU Manual), 6ml cena 8.793 Kč
AN512-10M CD5 (RTU Automated), 10ml cena 15.580 Kč
NU512-UC CD5 (Concentrated), 1ml cena Volejte Kč
Cytokeratin7 (clone EPR1619Y - Rabbit Monoclonal)
Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. The expression pattern in mice is similar to the reported human distribution, with substantial expression observed in lung, bladder, mesothelium, hair follicle, and ductal structures . Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma (p < 0.001). Studies provide further evidence that CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases . Also, the pattern of immunohistochemical expression of CK 7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas.
AN528-5M Cytokeratin7 (RTU Manual), 6ml cena 8.793 Kč
AN528-10M Cytokeratin7 (RTU Automated), 10ml cena 15.580 Kč
NU528-UC Cytokeratin7 (Concentrated), 1ml cena Volejte Kč
Ki67 (clone EPR3611 - Rabbit Monoclonal)
Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases. Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is considered as an important marker in human tumor diagnostics and prognostics.
AN527-5M Ki67 (RTU Manual), 6ml cena 8.793 Kč
AN527-10M Ki67 (RTU Automated), 10ml cena 15.580 Kč
NU527-UC Ki67 (Concentrated), 1ml cena Volejte Kč
ER alpha (clone EPR703(2) - Rabbit Monoclonal)
Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha.
AN509-5M ER alpha (RTU Manual), 6ml cena Volejte Kč
AN509-10M ER alpha (RTU Automated), 10ml cena Volejte Kč
NU509-UC ER alpha (Concentrated), 1ml cena Volejte Kč
Sarcomeric Actin (clone ZMSA-5 – Mouse Monoclonal)
This antibody is specific for alpha isoform of skeletal and cardiac muscle actin. It does not crossreact with alpha isoform of smooth muscle actin. This antibody shows wide cross reactivity to other animal species including sheep, rabbit, guinea pig, rat, frog and snake.
AM511-5M Sarcomeric Actin (RTU Manual), 6ml cena 7.551 Kč
AM511-10M Sarcomeric Actin (RTU Automated), 10ml cena 13.406 Kč
MU511-UC Sarcomeric Actin (Concentrated), 1ml cena Volejte Kč
p63 (clone 4B1E12 – Mouse Monoclonal)
p63 is a prostate basal cell marker, and expression is not observed in prostate tumors. As such, p63 is useful as part of a panel of antibodies for differential diagnosis of benign versus malignant prostate lesions. p63 may also be a marker for breast carcinoma and squamous cell carcinoma.
AM508-5M p63 (RTU Manual), 6ml cena 8.793 Kč
AM508-10M p63 (RTU Automated), 10ml cena 15.580 Kč
MU508-UC p63 (Concentrated), 1ml cena Volejte Kč
Villin (clone ID2C3 – Mouse Monoclonal)
Villin is a cytoskeletal protein of microvilli of epithelial cell brush border found in absorptive cells of the intestine and proximal renal tubes. Villin is a very specific marker for gastrointestinal tumors and adenocarcinomas of the pancreas. Other subsets of tumors stained with Villin are Merkel cell, lung (with rootlets), ovarian and kidney. It does not stain breast cancer. Used in a panel with CK7, CK20/CDX-2 and TTF-1,Villin can be very useful in differentiating colon adenocarcinoma from breast carcinoma and from lung Aden carcinoma.
AM525-5M Villin (RTU Manual), 6ml cena 7.551 Kč
AM525-10M Villin (RTU Automated), 10ml cena 13.406 Kč
MU525-UC Villin (Concentrated), 1ml cena Volejte Kč
p16[INK4a] (clone G175-405 – Mouse Monoclonal)
p16/INK4A is a tumor-suppressor protein and that genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation. The interaction of p16/INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. As such, expression of p16 INK4A is commonly associated with cellular senescence, and disruption of the p16 INK4A gene is frequently observed in human tumor. The p16/INK4A locus is deleted in a wide spectrum of tumors including melanoma, pancreatic adenocarcinoma, glioblastoma, certain leukemias, non-small cell lung cancer, cervical cancer, and bladder carcinoma.
AM560-5M p16[INK4a] (RTU Manual), 6ml cena Volejte Kč
AM560-10M p16[INK4a] (RTU Automated), 10ml cena Volejte Kč
MU560-UC p16[INK4a] (Concentrated), 1ml cena Volejte Kč
Glypican-3 (clone GPC3-88 – Mouse Monoclonal)
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. GPC3 belongs to a member of the glypican family of heparan sulfate proteoglycans. This protein may be involved in the suppression /modulation of growth in the predominantly mesodermal tissues and organs. Glypican-3 is thought to regulate tissue and organ growth through interactions with growth factors such as Insulin-like growth factor II or fibroblast growth factor 2. GPC3 was identified to be useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, and testicular germ cell tumors. A high level of GPC3 expression has also been found in some types of embryonic tumors, such as Wilms tumor and hepatoblastoma.
AM539-5M Glypican-3 (RTU Manual), 6ml cena 7.551 Kč
AM539-10M Glypican-3 (RTU Automated), 10ml cena 13.406 Kč
MU539-UC Glypican-3 (Concentrated), 1ml cena Volejte Kč
